Phone: 800-366-3773
en English
en Englishes Spanish

BRAF Mutation Analysis in Colorectal Cancers and Melanomas

Posted on April 21, 2022 by lincolndiagnosticsllcigfa

BRAF gene mutation testing is now considered critical in diagnosis, prognosis, treatment, and predicting patient outcome in response to targeted therapy for multiple cancer types. Mutations in BRAF have been reported in up to 60% of cutaneous melanomas and up to 18% of colorectal cancers. About 90% of these mutations are found to be V600E. Treatment with vemurafenib, a highly selective and potent inhibitor of BRAF V600E has shown promise in overall improvement and progression-free survival of patients with advanced melanoma with the V600E mutation. BRAF mutation testing has been […]

  • BRAF gene mutation testing is now considered critical in diagnosis, prognosis, treatment, and predicting patient outcome in response to targeted therapy for multiple cancer types.
  • Mutations in BRAF have been reported in up to 60% of cutaneous melanomas and up to 18% of colorectal cancers.
  • About 90% of these mutations are found to be V600E.
  • Treatment with vemurafenib, a highly selective and potent inhibitor of BRAF V600E has shown promise in overall improvement and progression-free survival of patients with advanced melanoma with the V600E mutation.
  • BRAF mutation testing has been suggested since its presence confers resistance to EGFR (cetuximab and panitumumab), when KRASis not mutated.

Result reported: Positive or Negative for V600E or V600K

Lincoln Labs BRAF Mutation Analysis offers:

  • Turnaround time of 7-10 days
  • Our highly qualified staff is accessible for any assistance regarding the tests.
  • Broad coverage by most private and public insurance.

Specimen Requirement:

  1. Formalin-fixed, paraffin-embedded (FFPE) tissues from routinely prepared small biopsies are practical and standard for BRAF mutation analysis.
  2. Specimens are optimally fixed in 10% neutral-buffered formalin, optimal fixation time ranges from 6 to 24 hours.
  3. Presence of tumor cells in the sample must be assessed by a pathologist.
  4. Ratio of tumor cells to normal cells is crucial for adequate mutation testing and should be provide by the pathologist.
  5. One H&E slide and 3-5 unstained slides (6-8microns ideal) are required for mutation testing.

Test Code:

ICD 10 Code:81210- BRAF (Raf proto-oncogene, serine/threonone kinase) (eg, colon cancer, melanoma), gene analysis, V600E variant(s).

This entry was posted in Uncategorized. Bookmark the permalink.

120 Responses to BRAF Mutation Analysis in Colorectal Cancers and Melanomas

  1. What’s Happening i’m new to this, I stumbled upon this I’ve found It positively useful and it has helped me out loads. I hope to contribute & assist other users like its helped me. Great job.

  6. Thank you for writing so many excellent articles. May I request more information on the subject?

  9. Thanks for posting. I really enjoyed reading it, especially because it addressed my problem. It helped me a lot and I hope it will help others too.

  14. May I request more information on the subject? All of your articles are extremely useful to me. Thank you!

  16. Thank you for your post. I liked reading it because it addressed my issue. It helped me a lot and I hope it will help others too.

  18. Thank you for your articles. They are very helpful to me. May I ask you a question?

  22. Thank you for posting such a wonderful article. It really helped me and I love the topic.

  23. Most of the things you state is astonishingly precise and that makes me wonder why I had not looked at this with this light previously. This particular article really did turn the light on for me as far as this subject goes. Well done.

  27. Thank you for your post. I really enjoyed reading it, especially because it addressed my issue. It helped me a lot and I hope it will also help others.

  28. Generally I do not read post on blogs, but I would like to say that this write-up very forced me to try and do so! Your writing style has been amazed me. Thanks, very nice article.

  29. Great post. I used to be checking constantly this blog and I am impressed! Very useful info specifically the last part 🙂 I take care of such information much. I used to be looking for this certain info for a very lengthy time. Thank you and best of luck.

  30. Thank you for writing about this topic. It helped me a lot and I hope it can help others too.

  31. Thank you for your post. I really enjoyed reading it, especially because it addressed my issue. It helped me a lot and I hope it will also help others.

  32. Aw, this was a really nice post. In idea I wish to put in writing like this moreover ? taking time and precise effort to make an excellent article? however what can I say? I procrastinate alot and certainly not appear to get something done.

  35. Thanks for the helpful posting. It is also my opinion that mesothelioma has an incredibly long latency time period, which means that signs of the disease would possibly not emerge until eventually 30 to 50 years after the 1st exposure to asbestos. Pleural mesothelioma, which is the most common variety and has effects on the area around the lungs, will cause shortness of breath, chest pains, as well as a persistent coughing, which may bring on coughing up blood.

  36. Simply wish to say your article is as amazing. The clearness in your submit is simply great and i can assume you are knowledgeable on this subject. Well along with your permission let me to grab your feed to stay up to date with impending post. Thank you a million and please carry on the gratifying work.

  37. Thank you for writing so many excellent articles. May I request more information on the subject?

  40. Hi, i think that i saw you visited my blog thus i came to ?return the favor?.I am trying to find things to improve my web site!I suppose its ok to use a few of your ideas!!

  41. May I request that you elaborate on that? Your posts have been extremely helpful to me. Thank you!

  42. You could definitely see your skills in the work you write. The world hopes for more passionate writers like you who are not afraid to say how they believe. Always follow your heart.

  44. Thank you for posting such a wonderful article. It helped me a lot and I adore the topic.

  46. I am so happy to read this. This is the type of manual that needs to be given and not the accidental misinformation that is at the other blogs. Appreciate your sharing this greatest doc.

  50. Someone essentially help to make seriously articles I would state. This is the very first time I frequented your website page and thus far? I amazed with the research you made to create this particular publish incredible. Wonderful job!

  52. I really enjoyed reading your post, especially because it addressed my issue. It helped me a lot and I hope it can help others too.

  55. Thank you for your post. I really enjoyed reading it, especially because it addressed my issue. It helped me a lot and I hope it will also help others.

  59. May I request more information on the subject? All of your articles are extremely useful to me. Thank you!

  61. What?s Happening i am new to this, I stumbled upon this I have found It absolutely useful and it has aided me out loads. I hope to contribute & help other users like its helped me. Great job.

  62. I?m impressed, I need to say. Actually hardly ever do I encounter a blog that?s each educative and entertaining, and let me let you know, you will have hit the nail on the head. Your thought is excellent; the difficulty is one thing that not sufficient people are speaking intelligently about. I’m very pleased that I stumbled throughout this in my seek for one thing regarding this.

  63. Can I simply say what a relief to seek out somebody who really is aware of what theyre speaking about on the internet. You positively know the best way to convey a difficulty to gentle and make it important. Extra people must learn this and perceive this facet of the story. I cant consider youre not more standard because you positively have the gift.

  64. I?ve read several excellent stuff here. Definitely price bookmarking for revisiting. I wonder how much effort you place to create any such magnificent informative site.

  65. Thanks for another informative blog. Where else could I get that kind of information written in such an ideal way? I’ve a project that I am just now working on, and I’ve been on the look out for such information.

  66. Thanks for the article. My partner and i have usually observed that almost all people are eager to lose weight because they wish to show up slim in addition to looking attractive. On the other hand, they do not constantly realize that there are more benefits for you to losing weight additionally. Doctors state that obese people experience a variety of illnesses that can be instantly attributed to their own excess weight. The good news is that people that are overweight in addition to suffering from various diseases can help to eliminate the severity of their particular illnesses by means of losing weight. It’s possible to see a progressive but noticeable improvement with health if even a slight amount of weight loss is accomplished.

  67. hey there and thank you for your info ? I have certainly picked up something new from right here. I am adding this RSS to my e-mail and can look out for much more of your respective intriguing content. Ensure that you update this again soon..

  68. whoah this blog is excellent i love reading your articles. Keep up the good work! You know, many people are searching around for this info, you could aid them greatly.

  69. You really make it seem so easy along with your presentation however I in finding this topic to be actually something which I believe I might by no means understand. It seems too complex and extremely large for me. I’m taking a look ahead in your subsequent post, I will attempt to get the hang of it!

  70. Tremendous issues here. I’m very satisfied to see your article. Thank you a lot and i am looking forward to contact you. Will you kindly drop me a e-mail?

  71. Generally I don’t read post on blogs, but I would like to say that this write-up very forced me to try and do it! Your writing style has been amazed me. Thanks, quite nice post.

  72. Wow, amazing weblog layout! How long have you ever been running a blog for? you made running a blog look easy. The full look of your website is wonderful, let alone the content material!

  75. Pretty nice post. I just stumbled upon your blog and wanted to say that I have really enjoyed browsing your blog posts. In any case I will be subscribing to your feed and I hope you write again soon!

  76. Pretty! This was a really wonderful post. Thank you for your provided information.

  77. I’ve been browsing on-line greater than three hours nowadays, yet I by no means found any fascinating article like yours. It is pretty value sufficient for me. In my view, if all web owners and bloggers made just right content as you probably did, the web shall be a lot more useful than ever before.

  78. Hey very nice blog!! Man .. Excellent .. Amazing .. I will bookmark your website and take the feeds also?I’m happy to find a lot of useful information here in the post, we need work out more strategies in this regard, thanks for sharing. . . . . .

  80. Just want to say your article is as astounding. The clarity in your post is simply excellent and i can assume you are an expert on this subject. Fine with your permission let me to grab your feed to keep updated with forthcoming post. Thanks a million and please keep up the enjoyable work.

  81. We are a group of volunteers and starting a new scheme in our community. Your site provided us with valuable information to work on. You have done a formidable job and our whole community will be grateful to you.

  82. It?s really a nice and useful piece of info. I?m glad that you shared this helpful info with us. Please keep us informed like this. Thanks for sharing.

  83. I’ve recently started a web site, the information you offer on this web site has helped me greatly. Thanks for all of your time & work.

  84. I would like to thnkx for the efforts you have put in writing this site. I am hoping the same high-grade site post from you in the upcoming also. In fact your creative writing skills has encouraged me to get my own blog now. Actually the blogging is spreading its wings fast. Your write up is a good example of it.

  85. Thanks for the sensible critique. Me & my neighbor were just preparing to do a little research on this. We got a grab a book from our area library but I think I learned more clear from this post. I am very glad to see such wonderful information being shared freely out there.

  86. Thanks for some other informative site. Where else may I am getting that kind of information written in such an ideal means? I’ve a venture that I am just now running on, and I’ve been at the look out for such information.

  87. A formidable share, I just given this onto a colleague who was doing a bit analysis on this. And he actually bought me breakfast because I found it for him.. smile. So let me reword that: Thnx for the treat! However yeah Thnkx for spending the time to discuss this, I really feel strongly about it and love reading extra on this topic. If doable, as you grow to be experience, would you thoughts updating your weblog with extra particulars? It is highly useful for me. Big thumb up for this blog put up!

  88. Youre so cool! I dont suppose Ive learn something like this before. So nice to search out anyone with some original thoughts on this subject. realy thanks for starting this up. this website is one thing that is wanted on the internet, somebody with somewhat originality. useful job for bringing something new to the internet!

  89. I appreciate, cause I found exactly what I was looking for. You’ve ended my four day long hunt! God Bless you man. Have a great day. Bye

  90. I loved as much as you will receive carried out right here. The sketch is tasteful, your authored subject matter stylish. nonetheless, you command get got an shakiness over that you wish be delivering the following. unwell unquestionably come more formerly again since exactly the same nearly very often inside case you shield this hike.

  91. Magnificent goods from you, man. I have understand your stuff previous to and you are just extremely excellent. I really like what you’ve acquired here, really like what you’re saying and the way in which you say it. You make it enjoyable and you still take care of to keep it sensible. I can’t wait to read much more from you. This is really a tremendous web site.

  92. Hey There. I found your blog using msn. This is a really well written article. I’ll be sure to bookmark it and come back to read more of your useful info. Thanks for the post. I will definitely comeback.

  93. I?ve been exploring for a bit for any high quality articles or blog posts in this kind of area . Exploring in Yahoo I ultimately stumbled upon this web site. Reading this information So i am happy to show that I’ve an incredibly just right uncanny feeling I came upon just what I needed. I so much for sure will make certain to don?t overlook this site and provides it a look on a continuing basis.

  95. Heya i?m for the first time here. I found this board and I in finding It truly helpful & it helped me out much. I’m hoping to give one thing again and help others like you aided me.

  96. Excellent goods from you, man. I’ve understand your stuff previous to and you are just too wonderful. I really like what you have acquired here, certainly like what you’re stating and the way in which you say it. You make it entertaining and you still care for to keep it smart. I can not wait to read far more from you. This is really a tremendous site.

  97. A lot of of what you mention happens to be supprisingly appropriate and that makes me ponder the reason why I hadn’t looked at this with this light previously. This article really did turn the light on for me as far as this particular subject matter goes. Nonetheless at this time there is actually 1 position I am not really too comfy with so while I attempt to reconcile that with the central theme of your point, permit me see what the rest of your visitors have to say.Nicely done.

  98. Excellent goods from you, man. I’ve understand your stuff previous to and you are just too wonderful. I actually like what you have acquired here, certainly like what you are saying and the way in which you say it. You make it entertaining and you still care for to keep it smart. I cant wait to read far more from you. This is really a great web site.

  99. The subsequent time I read a weblog, I hope that it doesnt disappoint me as a lot as this one. I mean, I do know it was my choice to learn, however I really thought youd have one thing interesting to say.

  100. you’re truly a excellent webmaster. The web site loading pace is incredible. It sort of feels that you are doing any unique trick. Also, The contents are masterpiece. you’ve performed a fantastic process in this matter!

  101. Thank you a lot for sharing this with all of us you actually realize what you are talking approximately! Bookmarked. Please additionally visit my website =). We could have a hyperlink trade contract between us!

  102. I was just looking for this info for a while. After six hours of continuous Googleing, finally I got it in your web site. I wonder what’s the lack of Google strategy that do not rank this type of informative websites in top of the list. Usually the top web sites are full of garbage.

  103. Thank you for another wonderful post. The place else may just anyone get that type of information in such a perfect approach of writing? I have a presentation next week, and I am at the search for such information.

  104. It’s perfect time to make a few plans for the long run and it is time to be happy. I’ve read this put up and if I could I want to recommend you some fascinating things or suggestions. Perhaps you could write next articles referring to this article. I desire to read more issues approximately it!

  105. I appreciate, cause I found exactly what I was looking for. You have ended my four day long hunt! God Bless you man. Have a nice day. Bye

  106. I would like to thank you for the efforts you’ve put in writing this website. I’m hoping the same high-grade website post from you in the upcoming also. In fact your creative writing skills has encouraged me to get my own web site now. Really the blogging is spreading its wings rapidly. Your write up is a great example of it.

  107. whoah this blog is magnificent i really like studying your articles. Stay up the good paintings! You already know, many persons are looking around for this info, you can help them greatly.

  108. Heya i?m for the first time here. I came across this board and I find It truly useful & it helped me out much. I hope to give something back and aid others like you aided me.

  109. I found your weblog website on google and verify a few of your early posts. Proceed to keep up the very good operate. I simply extra up your RSS feed to my MSN Information Reader. Seeking forward to reading more from you afterward!?

  110. wonderful submit, very informative. I wonder why the opposite experts of this sector don’t notice this. You should continue your writing. I’m sure, you’ve a huge readers’ base already!

  111. I think other web site proprietors should take this site as an model, very clean and fantastic user genial style and design, let alone the content. You’re an expert in this topic!

  112. Great paintings! This is the kind of information that should be shared across the web. Disgrace on the search engines for not positioning this submit upper! Thank you =)

  113. Hello there, I found your web site by way of Google while searching for a comparable topic, your website got here up, it appears great. I’ve bookmarked it in my google bookmarks.

  114. I have been browsing on-line more than 3 hours as of late, yet I by no means found any fascinating article like yours. It?s pretty value enough for me. In my opinion, if all webmasters and bloggers made just right content as you did, the web can be much more helpful than ever before.

  115. Thank you, I’ve just been looking for info about this subject for ages and yours is the greatest I have discovered till now.

  118. Thanks a bunch for sharing this with all people you really recognise what you’re talking approximately! Bookmarked. Please additionally consult with my web site =). We could have a hyperlink trade contract between us!

  119. Simply desire to say your article is as astonishing. The clarity in your post is just cool and i could assume you are an expert on this subject. Fine with your permission allow me to grab your RSS feed to keep updated with forthcoming post. Thanks a million and please keep up the enjoyable work.

  120. After examine a couple of of the blog posts in your web site now, and I really like your way of blogging. I bookmarked it to my bookmark website checklist and might be checking back soon. Pls take a look at my website online as well and let me know what you think.